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Congresso Brasileiro de Microbiologia 2023
 		Resumo: 430-1

430-1

USE OF RECOMBINANT MALATE DEHYDROGENASE (MDH) AND SUPEROXIDE DISMUTASE (SOD) [CU-ZN] AS ANTIGENS IN INDIRECT ELISA FOR DIAGNOSIS OF BOVINE BRUCELLOSIS

Autores:
Rafaella Silva Andrade (UFLA - UNIVERSIDADE FEDERAL DE LAVRAS) ; Angelica Rosa Faria (UNIFAL - UNIVERSIDADE FEDERAL DE ALFENAS) ; Helida Monteiro de Andrade (UFMG - UNIVERSIDADE FEDERAL DE MINAS GERAIS) ; Andrey Pereira Lage (UFMG - UNIVERSIDADE FEDERAL DE MINAS GERAIS) ; Elaine Maria Seles Dorneles (UFLA - UNIVERSIDADE FEDERAL DE LAVRAS)

Resumo:
The objective of this work was to validate an indirect enzyme-linked immunoassay (iELISA) using the recombinant proteins, malate dehydrogenase (MDH) and superoxide dismutase (SOD) [Cu-Zn], as antigens and to evaluate its ability to discriminate antibodies produced by vaccination from those induced by infection, in addition to its usefulness in the diagnosis of bovine brucellosis. Six groups were evaluated: G1 - Positive culture from animals (52 serum samples) naturally infected; G2 - non-vaccinated animals (28 serum samples) from selected from herds with a brucellosis, positive in RBT (Rose Bengal test) and 2ME (2-mercaptoethanol test); G3 - Animals from brucellosis-free area (32 serum samples); G4 - S19 vaccinated heifers (114 serum samples); G5 - Heifers vaccinated with RB51 (60 serum samples); G6 - Cross-reacting animals with inactivated Yersinia enterocolitica O:9 (42 serum samples). Diagnostic sensitivity (DSe) and diagnostic specificity (DSp) were estimated using the frequentist approach and the confidence interval (CI) (95%) calculated by the Clopper-Pearson (exact) method. The DSe estimates for iELISA_MDH in the G1 group was 63.46% (CI 95%: 48.96 - 76.38%) and for the G2 of 35.71% (CI 95%: 18.64 - 55.93%) and for the DSp were 50.00% in the G3 (CI 95%: 31.89 - 68.11%) and G4 58.77% (95% CI: 49.17 - 67.91%). Whereas for the iELISA_SOD estimates for DSe they were 67.31% in G1 (CI 95%: 52.89 - 79.67%) and in G2 it was 71.43% (CI 95%: 51.33 - 86.78%) and for DSp in G3 87.50% (CI 95%: 71.01 - 96.49%). iELISA_SOD exhibited could be used for the diagnosis of infected animals, increasing the range of serological tests available for the diagnosis of bovine brucellosis, with the advantage of being free of S-LPS. In contrast, the iELISA_MDH showed low usefulness as diagnostic test for bovine brucellosis, as well as for differentiate infection from vaccination.

Palavras-chave:
 Diagnosis, Diagnostic sensitivity, Diagnostic specificity, DIVA, Serology


Agência de fomento:
FAPEMIG, CAPES, CNPQ